Table 1 Primer information
Gene | Exon | Forward | Reverse | Amplicon size (bp) |
|---|---|---|---|---|
IFNGR1 | 1 | CGGTGACGGAAGTGACGTA | AACCGACGAGTTCAAACCAC | 372 |
2 | GGCAATGTGGCATCTTACAA | TGGGAAGGCTGATGAAAGAA | 292 | |
3 | CTTTCTCTGGCGTCTCCATC | TTTGGAGCATTCTGGGTTTC | 495 | |
4 | TGGTTTAAATGTGGTCCTGCT | TGATCTGTGAGTCTTGCTTGAA | 427 | |
5 | TGCATAGTATCGTGCTGTGTTG | TGCAAATGAGTTTGCTTTCC | 453 | |
6 | CCTAACTTCTGCTTCCTTCAGTG | GGCAGGTGACATCTTGTTGA | 389 | |
7-1 | GCCATTTGGTGGTCCATTAC | GGAGGTGGGGGCTTTTATTA | 599 | |
7-2 | GCAGCCATCTGACTCCAATAG | TCACTCCATTTGGTTGATACC | 489 | |
IFNGR2 | 1 | GAGCCGAATCCCCTCCAC | TGATCTGAGCACTCCGCATA | 401 |
2 | TGCCATTTCTGCACTTTGAC | AACCCGTGTGGTTAGACAGC | 496 | |
3 | GGGTGACAGAGCAAAACTCC | TTTAGGGTGACAGGCAGGAC | 482 | |
4 | TCGCACAACTGCACTCTAGC | CTCAGCCTCTATGGGGACAC | 489 | |
5 | TCTGAGACCTCACAGCCAGA | CTCATAAGCATTCCCCGATT | 473 | |
6 | TAAGCAGCATGGATGGAACA | AAAATTAGCAGGCCATGGTG | 429 | |
7 | CCAGCCTAGGCAAGAGTAAGA | CAGGGACAGGCCTTTTAGAG | 398 | |
STAT1 | 3 | TGTGTTTCGTGGAGGAGAAA | GTAAACATGGCCCCAAGTCA | 422 |
4 | CTGCTGCTAAAAGCAATGTCA | TGTGCTCAATTGTATTTGCTGA | 392 | |
5 | CCCGGAGAATATAAACAAATTCA | CTCCCACTTCTTGGGGCTAT | 327 | |
6 | ACAGTTTGCCAGCATTTTCC | GAGGTTTACACCCCAAGCAA | 351 | |
7 | CGTGTTTCTCTGGGTTCCAT | TAAAAGTCGGGGATGCTGTT | 405 | |
8 | GACTACAGGCGTCTGCCACT | TCATTCTCAACTGGGGCTCT | 448 | |
9 | GAGCCCCAGTTGAGAATGAA | TTCCAAGGGAGTGTTTCCAG | 420 | |
10 | CCATGATTCCCTTTAATCCAG | TTAAACCCTTGTAAATCATCTGAA | 323 | |
11 | GATCGTACTCTCTAACCCCCTTT | GCATTACATTTATGATTGGAAAAA | 439 | |
12 | CCTAAAACTGGAGGGGGAGT | AAATCTGCTGGGGTCATTTG | 410 | |
13 | GGCTGCAAATCCATAGGTGT | GCCCAAAATGTTGAACTTCC | 285 | |
14 | TGCAGAGATGTGAATGAGAGAAA | CAGTCGTGGGATAAGGAGGA | 407 | |
15 | GCCAATTTGTCCCATGTTCT | CAAATACCCAGCTCCTTTGC | 324 | |
16 | TGTGGCCTGTTGCAATGTTA | CATCAGCGATCTCAACTGGA | 400 | |
17 | TCCCCTGCTTTTAGTTGAGG | ACTGCCCTAGCTTACCCACA | 410 | |
18 | CCCCTACTGTGAAAGCACCT | TAGCAGAGGGGAAAAGAGCA | 348 | |
19 | CCATTTAAATTCCGCCCTCT | CCTTGGCCAGAGAAGAACAC | 382 | |
20 | TGTGCTGAATGGGACAGTTC | CTGAAGGGGCAGCCTATAAA | 357 | |
21 | CATTCCAGCCATTTTCTTGG | TTCATGTCCCAAACGCACTA | 340 | |
22 | CACTCAAATCCATCAACTCTGC | TCGAAAGCAAAACACTGCAT | 377 | |
23 | TGACTCAGGCAGGGAGGTTA | TTCAGCTGTGATGGCGATAG | 351 | |
24 | CACATCCCTGGAGTGTGAAA | CTCCAACTTGTCATGGCTCA | 481 | |
| Â | 25 | CCAGTGGGCAGCAGAGTAAT | TGCTGGCCTTTCTTTCATTT | 362 |