Fig. 4

The overexpression of WWTR1-AS1 upregulated the expression of Notch3

To test whether WWTR1-AS1 can sponge miR-136, the effects of WWTR1-AS1 and miR-136 overexpression on the expression nof Notch3, a target of miR-136, were analyzed by RT-qPCR (A) and Western blot analysis (B). Control (C) cells were untransfected cells. NC cells were miR-NC mimics - or empty vector-transfected cells. *, p < 0.05