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Figure 2 | BMC Women's Health

Figure 2

From: Fibroid explants reveal a higher sensitivity against MDM2-inhibitor nutlin-3 than matching myometrium

Figure 2

Western Blot analyses of explants treated with nutlin-3 reveal a concentration dependent increase of the amount of p53. A: Western Blot analysis of p53 of explants from an UL (case 700-1) treated with 30 μM and 50 μM nutlin-3 for 72 h shows a concentration-dependent increase of the amount of p53. Lane 1: marker SeeBlue Plus2 Pre-Stained Standard (Invitrogen, Karlsruhe, Germany), lane 2: control without nultin-3, lane 3: 30 μM nutlin-3, lane 4: 50 μM nutlin-3 (left to right). B: p53 protein expression determined after immunoblotting (c.f. A) by ImageJ (as described in the materials and methods section) against beta-actin. Control was set 100%. Ordinate: % change of p53 expression compared to control.

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BMC Women's Health

ISSN: 1472-6874

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