Figure 1

Human KIT organization and PCR amplification of its exons. A. Schematic representation of exon-intron map of human KIT. It is composed of 21 exons and 20 introns. Vertical bars and horizontal solid lines represent the exons and introns, respectively. The // indicates that these introns are not in scale. Arabic numbers above each of the vertical bars indicate the exon number. Modified from Giebel et al.[27]. B. Analysis of PCR products of the human KIT gene. Human genomic DNA spanning each of the human KIT exons were amplified using specific primer pairs. PCR products for each of the exons were separated by 1.5% agarose-electrophoresis and stained with ethidium-bromide. The Arabic numbers above each of the PCR products represent the exon number. DNA size markers (φ X174 RF DNA/HaeIII) are shown at the left of the panel (M).