Figure 5

Species-specific sequences of Neisseria gonorrhoeae opa genes. The electropherogram of gonococcal opa genes in this signature sequence region always illustrates a mixture of sequences generated by multiple opa gene templates. This particular tracing shows a clear base calling of 50 bases by the computer. However, in many cases only 40 bases on the right are selected for analysis because overlapping peaks tend to appear toward the left of the tracing due to co-migration of nucleotides having one molecular weight, but carrying different dye-labeled terminators due to the fact that gonococcal opa gene nested PCR products are not homogenous and may range from 127 to 130 base pairs in size.