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Figure 1 | BMC Women's Health

Figure 1

From: Prevalence of Trichomonas vaginalis infection among Egyptian women using culture and Latex agglutination: cross-sectional study

Figure 1

T. vaginalisin fresh wet mount examination × 40. The wet preparation of the vaginal discharge was made immediately, by applying a drop from the sample to a small area of a clean glass slide with a cover slip in order not to trap air bubbles. The whole smear was examined using conventional light microscopy for motile Trichomonas vaginalis with low power objective (× 10), then with high power objective (× 40). If motile flagellates with characteristic motility (jerky movement) and morphology of trichomonads were seen, the specimen was reported as positive for T. vaginalis. If no flagellated organisms were seen, the specimen was reported as negative for T. vaginalis. The test is dependent on demonstrating the motility of T. vaginalis that loses their motility rapidly after leaving the vaginal environment. Wet mount examination was performed within less than half an hour of collection in order to get optimal results and was held at room temperature. The T. vaginalis trophozoite is the oval as well as flagellated, or “pear” shaped as seen on a wet-mount.

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