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Figure 3 | BMC Women's Health

Figure 3

From: Prevalence of Trichomonas vaginalis infection among Egyptian women using culture and Latex agglutination: cross-sectional study

Figure 3

T. vaginalisin Modified Diamond culture × 40. Before inoculation, the culture tubes were removed from −20°C and incubated at 37°C for 1 to 2 hours to bring prepared medium at room temperature. Directly, the swab was inserted into the culture tubes containing the modified Diamond's medium. The tubes containing the inoculated medium were incubated vertically at 37°C in 5% CO2. The culture was examined daily using binocular or inverted microscope for the presence of T. vaginalis trophozoites. Aseptically, drop of the deposit was removed using a sterile pipette and placed on a slide and covered with a glass cover slip for wet mount examination. Examination was done under I0×-40× magnification. If the organism was not seen, the culture was incubated again for up to 1 week, with daily examination or every other day in the same manner. If no trichomonads were seen, the specimen was considered negative and discarded.

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