Prevalence of Trichomonas vaginalis infection among Egyptian women using culture and Latex agglutination: cross-sectional study

Table 1 Positive and negative results of symptomatic women using different test in comparison with the gold standard test (Diamond culture test)

Test used	M Diamond culture + ve-ve		Kappa value*	p value
Test used	( n = 30) (n = 970)		Kappa value*	p value
Kalon test + ve-ve	30 (100%)	20 (2.1%)	0.740	<0.001
Kalon test + ve-ve	0 (0%)	950 (97.9%)	0.740	<0.001
Giemsa test + ve-ve	10 (33.3%)	0 (0%)	0.492	<0.001
Giemsa test + ve-ve	20 (66.7%)	970 (100%)	0.492	<0.001
Wet mount + ve-ve	10 (33.3%)	0 (0%)	0.492	<0.001
Wet mount + ve-ve	20 (66.7%)	970 (100%)	0.492	<0.001

*a measure of agreement between the test and modified Diamond culture.
As shown in the table above, 30 symptomatic women were positive for trichomonas infection using the gold standard test. These were also positive using the Kalon Latex test, however the Kalon test detected another 20 cases as positive for trichomonas infection which were however culture negative. However, both Giemsa stain and wet mount detected the same 10 cases that were culture positive accounting for 33.3% of total cases detected by the gold standard reference test. The relation between the results obtained using the gold standard test in comparison to the other tests applied was statistically significant for each test with a strong agreement (kappa = 0.740) for the Kalon test and moderate agreement with culture for the Giemsa stain and wet mount(kappa = 0.492).

ISSN: 1472-6874