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Table 2 Diagnostic performance of Mount Microscopy and JD’s Trichomonas V® Rapid Antigen Assay compared to PCR in the detection of T. vaginalis among females

From: Trichomonas vaginalis infection and the diagnostic significance of detection tests among Ghanaian outpatients

Diagnosticsa

PCR

Testa

Percent sensitivity (95%CI)

Percent Specificity (95%CI)

Predictive values

Likelihood ratio

Cohen’s Kappa values (95%CI)

Pos.; Neg.

Positive

(95%CI)

Negative

(95%CI)

Positive

Negative

Wet Mount Microscopy

18/24; 87/126

31.6

(20.3, 45.3)

93.5

(85.9, 97.3)

75.0

(52.9, 89.4)

69.1

(60.1, 52.5)

3.0

(1.4, 6.2)

0.45

(0.34, 0.59)

0.283(70.0%)

(0.15, 0.42)

 Females

18/24; 58/86

39.2

(25.4, 54.6)

90.6

(80.0, 96.1)

75.0

(52.9, 89.3)

67.4

(56.3, 76.9)

3

(1.4, 6.2)

0.48

(35.2, 66.1)

0.313

(0.151, 0.482)

 Males

0/0; 29/40

0

100

(85.4, 100)

72.5

(55.8, 84.8)

0.37

(0.23–0.64)

0.00

 Symptomatic patients

14/20; 28/61

33.3

(20.0, 49.6)

86.7

(72.5, 94.4)

70.0

(45.6, 87.1)

54.1

(40.9, 66.7)

2.3

(1.1, 4.8)

0.85

(62.8, 1.1)

0.109

(−0.057, 0.276)

 Asymptomatic patients

4/4; 59/65

40.0

(13.6, 72.6)

100

(92.3, 100)

100

(39.5, 100)

0.9

(80.3, 96.1)

0.10

(0.05–21.8)

0.533

(0.217,0.849)

 Antigen Test

15/19; 87/132

25.0

(15.1, 38.1)

96.7

(89.8, 99.1)

83.3

(57.7, 95.5)

65.9

(57.1, 73.7)

5.0

(1.7, 14.3)

0.52

(0.41, 0.66)

0.233

(0.103, 0.364)

 Females

13/15;57/95

25.4

(14.73, 9.9)

96.6

(87.2, 99.7)

86.6

(58.3,97.6)

0.6

(49.4, 69.7)

6.5

(1.78, 23.9)

0.67

(0.51, 86.4)

0.232

(0.096, 0.368)

 Males

3/4; 29/36

0.3

(0.8, 64.3)

96.7

(80.9, 99.8)

75.0

(21.9, 98.6)

80.5

(63.4, 91.2)

3

(0.51, 17.9)

0.24

(0.12, 0.48)

0.333

(−0.001, 0.667)

 Asymptomatic patients

3/4; 55/65

23.1

(6.1, 54.0)

98.2

(89.1, 99.9)

75.0

(21.9, 98.6)

84.6

(73.1, 91.9)

3

(0.50, 0.18)

0.18

(0.10, 32.3)

0.290

(0.004, 0.576)

 Symptomatic patients

13/15; 28/66

25.4

(14.7, 39.9)

93.3

(76.4, 98.8)

86.6

(58.3, 97.6)

42.4

(30.5, 55.1)

6.5

(1.7, 23.9)

1.4

(1.1, 1.7)

0.151

(0.024, 0.278)

  1. aPos. (a/b), true positives as confirmed with PCR/total positives from diagnostic test; Neg. (c/d), true negatives as confirmed with PCR/total negatives from diagnostic test; CI confidence interval, PCR polymerase chain reaction