From: Androgen receptor expression in a Sri Lankan patient cohort with early breast carcinoma
Marker | Supplier | Clone | Antigen retrieval | Primary dilution | Amplification step | Interpretation | Binarized as positive | Binarized as negative |
---|---|---|---|---|---|---|---|---|
ER | Dako | M7047 Clone ID5 | Pressure cooker in citrate buffer | 1:60 | Universal secondary antibody Envision (Dako K5007) | ASCOCAP 2010 guideline [22] | > = 1% of tumour cells positive | < 1% of tumour cells positive |
PR | Dako | M3569 Clone PgR636 | Pressure cooker in citrate buffer | 1:50 | ASCOCAP 2010 guideline [22] | > = 1% of tumour cells positive | < 1% of tumour cells positive | |
HER2 | Dako | A0485 | Microwave in citrate buffer | 1:600 | ASCOCAP 2013 guideline [23] | HER2 = 3+ | HER2 = 0,1 | |
CK5/6 | Dako | M7237 Clone D5/16B4 | Pressure cooker in citrate buffer | 1:25 | Proportion Score 0 = No cytoplasmic staining 1-Positive cytoplasmic staining in 1–33% of cells 2-Positive cytoplasmic staining in 33–66% of cells 3-Positive staining in the cytoplasm in > 66% of cells Intensity Score 0-No cytoplasmic staining 1-Weak cytoplasmic staining 2-Moderate cytoplasmic staining 3Strong cytoplasmic staining | Total score ≥ 2 | Total score < 2 | |
34βE12 | Dako | N1553 Clone 34βE12 | Microwave in Dako Target Retrieval Solution (S1700) | Undiluted | ||||
CK14 | Cell Marque | LL002 | Pressure cooker in citrate buffer | 1:100 | ||||
EGFR | Dako | M7239 Clone E30 | Proteolytic digestion with Dako Proteinase K (S3020) | 1:25 | Atkins [24] et al. | Any membrane staining above background, whether or not it is completely circumferential. | No membrane staining above background in any tumor cell | |
AR | Dako | M3562 Clone AR 441 | Pressure cooker in citrate buffer at pH 6 for 7–8 min | 1:50 | ASCOCAP 2010 guideline on immunohistochemical testing of ER/PR [22] | > = 1% of tumour cells positive | < 1% of tumour cells positive |