Fig. 3

Exosomal tRF-Leu-AAG-001 derived from mast cells in ectopic tissue. A Nucleic acid electrophoresis showed that tRF-Leu-AAG-001 was highly expressed in ectopic tissues (*p = 0.0116), while the expression of tRF-Leu-TAG-015 had no statistically difference between the EMs group (n = 6) and the NE group (n = 7). Two-tailed Student’s t-test was used. B There was no significant difference in the expression of tRF-Leu-AAG-001 in the primary ectopic endometrial cells (n = 10) and the primary normal endometrial cells (n = 10).Two-tailed Student’s t-test was used. (The original images of the gels were shown in the Additional file 4: Figures). C In the 3D cell spheroid model, RNA probes and fluorescence immunolocalization showed that tRF-Leu-AAG-001 was highly expressed in mast cells of the ectopic cell spheroid (200*). Anti-CD117 was the marker of mast cells; DAPI was used for nuclear staining. NE group: normal endometrial group; EC group: ectopic cells