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Fig. 4 | BMC Women's Health

Fig. 4

From: Exosomal tRF-Leu-AAG-001 derived from mast cell as a potential non-invasive diagnostic biomarker for endometriosis

Fig. 4

Regulation of inflammatory factors and angiogenesis by tRF-Leu-AAG-001. A According to the analysis of gel electrophoresis results, the histogram showed that luva cell line (n = 3) highly expresses tRF-Leu-AAG-001 (***p < 0.001). Two-tailed Student’s t-test was used. (The original images of the gels were shown in the Additional file 4: Figures). B Exosomes labeled with PKH67 (Exo-PKH67) were visible in HUVECs after 24 h of incubation. The exosomes were stained green by PKH67 and the nuclei were stained blue by DAPI.Scale bar = 25 μm. C The expressions of IL-6, IL-10, IL-1β, and TNF-α were significantly decreased luva cell line (n = 3) after tRF-Leu-AAG-001 was knockdown by siRNA (**** p < 0.0001).Two-tailed Student’s t-test was used. D The tube formation displayed after exosomal tRF-Leu-AAG-001 derived from luva was able to induce angiogenesis (**p < 0.01, ****p < 0.0001).Two-tailed Student’s t-test was used

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