Fig. 2From: Kisspeptin regulates the proliferation and apoptosis of ovary granulosa cells in polycystic ovary syndrome by modulating the PI3K/AKT/ERK signalling pathwayKisspeptin promotes the proliferation of ovarian granulosa cell line KGN. A–E KGN cells were transfected with KISS-1-overexpressing vectors or the empty vector. The RNA (A) and protein (B) levels of kisspeptin were measured. Cell proliferation and cell cycle progression was assessed using CCK-8 (C), EdU (D), and flow cytometry (E). F–J KGN cells were transfected with KISS-1 shRNAs or the scrambled shRNA. The RNA (F) and protein (G) levels of kisspeptin were measured. Cell proliferation and cell cycle progression were assessed with CCK-8 (H), EdU (I), and flow cytometry (J). n.s, not significant; *P < 0.05, **P < 0.01, ***P < 0.001Back to article page