Fig. 3

Kisspeptin inhibits the apoptosis of ovarian granulosa cell line KGN. A, B KGN cells were transfected with KISS-1-overexpressing vectors or the empty vector. Cell apoptosis (A) and protein levels of Bcl-2, Bax, and cleaved-caspase 3 (C-caspase-3) (B) were detected by flow cytometry and western blotting. C, D KGN cells were transfected with KISS-1 shRNAs or the scrambled shRNA. Cell apoptosis (C) and protein levels of Bcl-2, Bax, and cleaved-caspase 3 (C-caspase-3) D were detected by flow cytometry and western blotting. *P < 0.05, **P < 0.01, ***P < 0.001