Fig. 4

Kisspeptin inhibits oxidative stress of ovarian granulosa cell line KGN. A–E KGN cells were transfected with KISS-1-overexpressing vectors or the empty vector. The intracellular levels of ROS (A), MDA (B), and antioxidant enzymes GSH (C), SOD (D), and CAT (E) were measured. F–J KGN cells were transfected with KISS-1 shRNAs or the scrambled shRNA. The intracellular levels of ROS (F), MDA (G), and antioxidant enzymes GSH (H), SOD (I), and CAT (J) were measured. *P < 0.05, **P < 0.01