Table 2 qPCR reaction mixture used for amplification of ICAM-1, TNF-α, MCP-1, PAI-1 and β-actin Gene
Reagent | Concentration | Volume |
|---|---|---|
Nuclease free water | 8.9 µl | |
Forward primer | 10 pmol/µl | 0.3 µl |
Reverse primer | 10 pmol/µl | 0.3 µl |
cDNA sample | 70 ng/µl | 0.5 µl |
SYBR Green(MM) | 2 X | 10 µl |
Total volume | 20 µl |