Fig. 2

PCGEM1 stimulates endometrial stromal cell proliferation by activating the ANTXR2 pathway. (A) qPCR analysis of PCGEM1 expression in endometrial stromal cells (ESCs) after transfection with si-PCGEM1 for 24 h (n = 3). (B-D) Clone formation and CCK-8 assays were performed to assess normal endometrial stromal cell (NESC) and ESC proliferation after transfection (n = 3). (E-F) A Transwell assay was performed to evaluate the migration capacity of NESCs and ESCs (400× magnification) (n = 3). (G) A tube formation assay was carried out to evaluate the tube formation ability of NESCs and ESCs (n = 3). (H-I) Relative protein expression levels of ANTXR2 and its downstream proteins (including YAP1, COL5A2 and VEGFC) in NESCs and ESCs (n = 3). The membranes were cut prior to incubation with antibodies after protein transfer from the same gel. Scale bar = 20 μm. Mean ± SD from three independent tests. *P < 0.05, **P < 0.01, ***P < 0.001