Fig. 3

ANTXR2 is a target of miR-124-3p. (A) Prediction of the miR-124-3p binding sequence in the 3’UTR of ANTXR2. (B) Relative expression of miR-124-3p mRNA in different tissues (n = 3). (C) A dual luciferase reporter assay was used to investigate the relationship between miR-124-3p and ANTXR2 (n = 3). (D-E) An RNA pull-down assay was performed to evaluate the interaction between ANTXR2 and miR-124-3p (n = 3). The regions of the membrane corresponding to AGO2 and GAPDH were cut prior to incubation with antibodies after protein transfer from the same gel. (F-G) qPCR and immunoblotting analyses were adopted to verify the relationship between ANTXR2 and miR-124-3p expression (n = 3). Mean ± SD from three independent tests. *P < 0.05, **P < 0.01, ***P < 0.001