Figure 2
From: Investigation of KIT gene mutations in women with 46,XX spontaneous premature ovarian failure
SSCP analysis of KIT PCR products The PCR products were separated on 20% TBE-acrylamide gel electrophoresis and stained with ethidium bromide. SSCP analysis of samples from three normal control women and three patients with spontaneous premature ovarian failure are shown for exon 10,16, and 17. DNA mobility of PCR products from exons 10 and 16 was altered in one patient (Lane 4). One patient showed an alternation of DNA mobility in the PCR product from exon 17 (Lane 5). Also shown is the DNA mobility of samples from normal women (Lanes 1–3) and from a representative patient with no changes in DNA mobility (Lane 6).