Table 1 Primer sets and conditions for amplification of human KIT exons. Forward and reverse primers flanking each of the 21 exons are shown. The size of each exon-coding region, the size of each PCR product generated by each set of primers, and the respective annealing temperatures are indicated. Sequences marked by a * are derived from Spritz et al..[28]
From: Investigation of KIT gene mutations in women with 46,XX spontaneous premature ovarian failure
Exon No | Exon # Size (bp) | Primers | PCR product Size (bp) | Annealing Tm(°C) |
|---|---|---|---|---|
| Â | Â | Forward Reverse | Â | Â |
1 | 88(67) | 5'GGACCAGAGCTCGGATC3' / 3'AGTCCTCTCTCCGGATG5' | 198 | 62 |
2 | 270 | 5'CTCAGTATTGGAAGAAGTGC3' / 3'TATCTATGGCTCAGTCATCC5' | 438 | 55 |
3 | 282 | 5'GCTTCTATAGATCCTGCC3' / 3'GATCAACGAGAAGAGAAGTC5' * | 367 | 55 |
4 | 137 | 5'TGTACACATTTGAGGAGAAA3' * / 3'CTGACAGACGCACTAGTCG5' * | 330 | 55 |
5 | 169 | 5'TGGAGAAGTTAATTGCTGCT3' * / 3'TGTGCTTTCATTGCAAGAGG5' | 435 | 55 |
6 | 190 | 5'TTGTAATTCCAAGATGAGG3' * / 3'TACTAGGATGAGGACATAGG5' | 422 | 55 |
7 | 116 | 5'TATGTGTGTGCGTGTTTATG3' * / 3'CAAGTTGAGTCCTTGCAGCTG5' * | 372 | 62 |
8 | 115 | 5'CTCCTTGGTTCAGATTCTGC3' / 3'GTGAATTGCAGTCCTTCC5' | 321 | 55 |
9 | 194 | 5'TATGCCACATCCCAAGTG3' / 3'GGTGTGATGCATGTATTACC5' | 390 | 55 |
10 | 107 | 5'ACATAGCTTTGCATCCTGC3' / 3'ATTGTCTCAGTCATTAGAGCAC5' * | 280 | 55 |
11 | 127 | 5'GAGTGCTCTAATGACTGAG3' / 3'CCACTGGAGTTCCTTAAAG5' | 266 | 56 |
12 | 105 | 5'ATGGTCCTTCAATTCCACC3' / 3'TTCTGTCAAATGGGCACTC5' | 270 | 55 |
13 | 111 | 5'GACATCAGTTTGCCAGTTG3' / 3'GCAAGAGAGAACAACAG5' | 296 | 58 |
14 | 151 | 5'GACTAAGTAGTCTGATCC3' / 3'ACCCCATGAACTGCCTGTC5' * | 327 | 55 |
15 | 92 | 5'TAGAGCATGACCCATGAG3' / 3'ACCCACTTGCAACCCTAACT5' * | 325 | 60 |
16 | 128 | 5'GGTATGTCATTGCCACTG3' / 3'GGCTCTAAAATGCTCTGTTCT5' * | 296 | 55 |
17 | 123 | 5'GTGAACATCATTCAAGGCG3' / 3'TTACATTATGAAAGTCACAGG5' * | 390 | 55 |
18 | 112 | 5'CACATTTCAGCAACAGC3' / 3'CCTTCCTTGATCATCTTGT5' * | 382 | 53 |
19 | 100 | 5'CTCAGAGCATCTTCTTGAAG3' / 3'ACATCTGGGTTTCTGTCTC5' | 252 | 58 |
20 | 106 | 5'CCATATGTCCAGTTGCATAG3' / 3'TACCTGAAGCCCAATTTGC5' | 257 | 60 |
21 | 2407(129) | 5'TGGCCACAAAGTTCTTGG3' / 3'TATCCTGGAGTTGGATGC5' | 369 | 55 |