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Table 2 Antibodies used for characterization of the Tumors

From: Long-term multidisciplinary treatment including proton therapy for a recurrent low-grade endometrial stromal sarcoma and pathologically prominent epithelial differentiation: an autopsy case report

Antibody Source
anti-cytokeratin (clone: CAM5.2, mouse) Becton, Dickinson and Company BD Biosciences, San Jose, CA, USA
anti-CD56 monoclonal (clone: 1B6, mouse) Nichirei Bioscience Co. Ltd. Tokyo, Japan
anti-chromogranin A polyclonal (rabbit) Nichirei
anti-human Ki-67 monoclonal (clone MIB-1, mouse) Agilent Technologies, Santa Clara, CA, USA
anti-CD10 monoclonal (clone: 56C6, mouse) Nichirei
anti-vimentin monoclonal (clone V9, mouse) Nichirei
anti-estrogen receptor α monoclonal (clone EP-1, rabbit) Agilen
anti-progesterone receptor monoclonal (clone PgR636, mouse) Agilent
anti-CD99 monoclonal (clone O13, mouse) F.Hoffmann-La Roche Ltd., Basel Switzerland
anti-inhibin α monoclonal (clone R1, mouse) Agilent
anti-cyclin D1 monoclonal (clone SP4-R, rabbit) Ventana Medical Systems, inc. Tuscon, AZ, USA
  1. Methods of immunohistochemistry: Tissue sections were cut to 4 μm thick and immunohistochemically stained using paraffin sections of surgical specimens. Heat-induced epitope retrieval was performed by heating deparaffinized sections in buffer (Nichirei Histofine, pH 9.0) (Nichirei Bioscience Co. Ltd. Tokyo, Japan) for 30 min at 98 °C for CAM5.2, CD56, Ki-67, CD10, estrogen receptor, progesterone receptor, CD99, inhibin α, and cycline D1. The slides were developed using 3,3′-Diaminobenzidine and were counterstained with hematoxylin. All antibodies were used at a dilution of 1:50