Table 1 Primers and PCR settings used for genotyping the cytokines polymorphisms
TNF-α (rs1800629) | IL-1β (rs1143634) | IL-1Ra (rs2234663) | |
|---|---|---|---|
Primers | F:AGGCAATAGGTTTTGAGGGCCAT | F:CTCAGGTGTCCTCGAAGAAATCAAA | F:TCCTGGTCTGCAGGTAA |
R:TCCTCCCTGCTCCGATTCCG | R:GCTTTTTTGCTGTGAGTCCCG | R:CTCAGCAACACTCCTAT | |
Cycles | 35 | 35 | 35 |
Initial denaturation | 94 °C for 5 min | 94 °C for 5 min | 96 °C for 1 min |
Denaturation | 94 °C for 30 s | 94 °C for 30 s | 94 °C for 1 min |
Annealing | 60 °C for 30 s | 55 °C for 30 s | 62 °C for 1 min |
Elongation | 72 °C for 30 s | 72 °C for 30 s | 72 °C for 1 min |
Final extension | 72 °C for 5 min | 72 °C for 5 min | 72 °C for 7 min |