Fig. 1

MT limits SS-induced KGN cell autophagy and downregulates miR-15a-5p. A The expression levels of miR-15a-5p in the serum of the healthy controls (n = 40) and POI patients (n = 32) were examined using qRT-PCR. KGN cells were treated with serum starvation (SS) to induce autophagy and then treated with melatonin (MT). B KGN cell viability was detected via MTT assay; C The expression levels of autophagy-related proteins Beclin 1, LC3 II/I, and p62 in KGN cells were examined via Western blotting; D The expression levels of miR-15a-5p in KGN cells were examined via qRT-PCR. Experiments were performed in 3 independent repetitions; experimental data were presented as mean ± SD; data in figure A were examined via the t-teat; data in figure B were examined via two-way ANOVA; data in figures C-D were examined via one-way ANOVA, followed by Tukey’s post-hoc test; ** p < 0.01