Fig. 2

miR-15a-5p upregulation boosts SS-induced KGN cell autophagy. KGN cells were transfected with plasmid miR-15a-5p-mimic (miR-mimic), with mimic-NC as a control. A The transfection efficiency was examined via qRT-PCR. KGN cells with miR-15a-5p-mimic were combined with MT treatment. B KGN cell viability was detected via MTT assay; C The expression levels of autophagy-related proteins Beclin 1, LC3 II/I, and p62 in KGN cells were examined via Western blotting. Experiments were performed in 3 independent repetitions; experimental data were presented as mean ± SD; data in figure A were examined via Student’s t-test and data in figures B-C were examined via one-way ANOVA, followed by Tukey’s post-hoc test; * p < 0.05 and ** p < 0.01