Fig. 3

miR-15a-5p binds to Stat3. A The binding association between miR-15a-5p and Stat3 was verified via dual-luciferase reporter gene assay; B-C Stat3 expression levels in KGN cells were examined via qRT-PCR and Western blotting; D The expression levels of Stat3 in the serum of the healthy controls (n = 40) and POI patients (n = 32) were examined using qRT-PCR; E The correlation between serum miR-15a-5p expression and serum Stat3 expression was analyzed by Pearson correlation analysis. Experiments were performed in 3 independent repetitions; experimental data were presented as mean ± SD; data in figure A were examined via two-way ANOVA and data in figures B-C were examined via one-way ANOVA, followed by Tukey’s post-hoc test; ** p < 0.01