Fig. 4

Silencing Stat3 neutralizes the repression of MT on SS-induced KGN cell autophagy. KGN cells were transfected with si-Stat3-1 and si-Stat3-2, with si-NC as a negative control. A The transfection efficiency was examined via qRT-PCR. si-Stat3-1 with better silencing effect was chosen for combined treatment with MT. B The protein levels of Stat3 in KGN cells were determined via Western blotting; C KGN cell viability was detected via MTT assay; D The expression levels of autophagy-related proteins Beclin 1, LC3 II/I, and p62 in KGN cells were examined via Western blotting. Experiments were performed in 3 independent repetitions; experimental data were presented as mean ± SD; data in figure C were examined via two-way ANOVA and data in figures A-B and D were examined via one-way ANOVA, followed by Tukey’s post-hoc test; ** p < 0.01