Fig. 6

Silencing the PI3K-Akt-mTOR pathway promotes SS-induced KGN cell autophagy. KGN cells were treated with PI3K inhibitor (LY294002) and combined with MT treatment. A The activation of the PI3K-Akt-mTOR pathway in KGN cells was detected via Western blotting; B KGN cell viability was detected via MTT assay; C The expression levels of autophagy-related proteins Beclin 1, LC3 II/I, and p62 in KGN cells were examined via Western blotting. Experiments were performed in 3 independent repetitions; experimental data were presented as mean ± SD; data in figure B were examined via two-way ANOVA and data in figures A and C were examined via one-way ANOVA, followed by Tukey’s post-hoc test; ** p < 0.01