Fig. 3

The effect of Pentamidine on the expression of p-AKT, MMP-2 and MMP-9 proteins in EC cells. A Ishikawa cells were treated with or without indicated concentrations of Pentamidine for 24 h. Protein levels of AKT and p-AKT were analyzed by Western blotting. GAPDH was used as the loading control. B Densitometric quantification of Western blot data presented in panel A. Data are represented as mean ± SD from three independent experiments. *P < 0.05. C Ishikawa cells were treated with or without indicated concentrations of Pentamidine for 24 h. Protein levels of MMP-2 and MMP-9 were analyzed by Western blotting. GAPDH was used as the loading control. D, E Densitometric quantification of Western blot data presented in panel C. Data are represented as mean ± standard deviation from three independent experiments. *P < 0.05. GAPDH was used as the loading control. F Ishikawa cells were respectively transfected with empty vector and HA-CA-AKT construct, and then treated with Pentamidine (15 μmol/L) or vehicle for 24 h. Protein levels of HA-tag, MMP-2 and MMP-9 were analyzed by Western blotting. GAPDH was used as the loading control. The blot (HA) was cut prior to hybridisation with antibodies during blotting