Fig. 2

Western blot quantification of PTEN protein in pre- and post-pubertal human ovaries. A Patients that received chemotherapy before cryopreservation. B Patients that did not receive chemotherapy before cryopreservation. C PTEN expression level comparison between the chemotherapy-treated and untreated group. The bars represent the values expressed as mean ± ES. Different letters indicate significant differences between groups (Tukey, α: 0.05, p < α). Group 1, treated-patients; Group 2, untreated-patients. Numbers over lanes and under bars in A and B indicate the patient number (see Table S1). HUF, Human Uterine Fibroblast cell line. Original, full-length gels from where clipped bands were extracted are shown in Fig. 1S