Table 1 The primers sequence Bax, Bcl2, GAPDH of genes
Gene name | Sequence | Product size (bp) | annealing temp |
|---|---|---|---|
Bcl2 | For: 5’- GCAAACTGGTGCTCAAGG − 3’ Rev: 5’- CAGCCACAAAGATGGTCA − 3’ | 183 | 56.63 |
Bax | For: 5’- GAGTGGGATACTGGAGATGAAG − 3’ Rev: 5’- TGGTAGCGACGAGAGAAGTCC − 3’ | 233 | 57.4 |
GAPDH | For: 5’- AAGTTCAACGGCACAGTCAAGG − 3’ Rev: 5’- CATACTCAGCACCAGCATCACC − 3’ | 121 | 61.58 |