Fig. 6
From: Metabolism-associated molecular classification of cervical cancer
Validation of key genes in the CESC. A Knockdown decreased the expression level of KALRN protein. Total protein extracted from Hela cell treated with an siRNA against KALRN (si-KALRN) or with a negative control siRNA(si-NC) for 3 days, analyzed by western blotting with antibodies against the indicated proteins (KALRN and β-actin). B The fluorescence intensity of knockdown KALRN decreased. The cells were transfected with siRNA or with a negative control si-NC. After 48 h, the cells were collected for immunofluorescence to detect the change of fluorescence intensity and protein localization. Green indicates KALRN, blue indicates DAPI staining, indicating nucleus; Merge is the overlying of two colors. The scale bar 20 μm. C CCK-8 assays were conducted to examine Hela cell viability after the knockdown of KALRN. D RT-qPCR was used to detect the expression of KALRN, HERC1 and CURB