Fig. 1

Fancd2 was up-regulated in endometrial cancer and associated with chemoresistance. A RT-qPCR was used to detect Fancd2 expression in normal and endometrial cancer (EC) tissues. B Western blot was adopted to check the expression level of Fancd2 in the Normal group and EC group. C RT-qPCR was employed to detect Fancd2 expression in human endometrial epithelial cells (hEEC) and EC cells (Ishikawa). D Western blot was adopted for detecting Fancd2 expression levels in hEEC and Ishikawa cells. E RT-qPCR was used for measuring the transfection efficiency of Fancd2 in Ishikawa cells. F Western blot was used to examine the expression level of Fancd2 in the Ishikawa cells in Vector group, Fancd2 group, siNC group and si-Fancd2 group. G–J MTT was utilized to test the cell activity of Ishikawa cells treated with different concentrations of paclitaxel (also known as taxol) (G), cisplatin (H), doxorubicin (I), and sorafenib (J). * P < 0.05, ** P < 0.01