Fig. 2
From: Interferon-γ-induced GBP1 is an inhibitor of human papillomavirus 18
GBP1 inhibits HPV by degrading the E6 protein. Co-transfected pcDNA3.1-GBP1-EGFP and pcDNA3.1-E6-RFP into HEK293T cells in a 3:1 ratio. After 48 h transfected, the fluorescence signals from pcDNA3.1-GBP1-EGFP or pcDNA3.1-E6-RFP were observed under invert fluorescence microscope (a). The intensities of fluorescence from pcDNA3.1-GBP1-EGFP or pcDNA3.1-E6-RFP were detected by multifunctional microplate reader and expressed as relative fluorescence units (RFU) compare to the control group (b). (c) cells were harvested and the protein levels of GBP1 and E6 were determined by Western Blot using GAPDH as an internal reference. blank control (-), (mock) 1.5 µg pcDNA3.1 vector and 0.5 µg pcDNA3.1-E6-RFP were transfected, (control) 1.5µg pcDNA3.1 vector and 0.5µg pcDNA3.1-RFP or 1.5µg pcDNA3.1-EGFP and 0.5µg pcDNA3.1 vector were transfected, (1) 1.5 µg pcDNA3.1-GBP1-EGFP and 0.5 µg pcDNA3.1-E6-RFP were transfected. Data were shown as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01