Fig. 4
From: Interferon-γ-induced GBP1 is an inhibitor of human papillomavirus 18
HPV E6 protein degrades GBP1 via the proteasome pathway. pcDNA3.1-GBP1-EGFP and pcDNA3.1-E6-RFP were co-transfected into HEK293T cells at a ratio of 1:3, and the control group was transfected with pcDNA3.1-EGFP or pcDNA3.1 vector (mock). 36 h after transfection, Mg132 (1:1000) and BAF-A1 (1:1000) were added, and the control group was added with an equal ratio of DMSO. After 48 h of transfection, the fluorescence signals from pcDNA3.1-GBP1-EGFP were observed (a). The intensities of fluorescence from pcDNA3.1-GBP1-EGFP were detected (b). (c) The expression of GBP1 and E6 protein was detected by Western Blot. (1,3,5) 1.5 µg pcDNA3.1 vector and 0.5 µg pcDNA3.1-GBP1-EGFP were transfected. (2,4,6) 1.5 µg pcDNA3.1-E6-RFP and 0.5 µg pcDNA3.1-GBP1-EGFP were transfected. Data were shown as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01