Table 1 Source and dilutions of the antibodies and epitope retrieval methods used in this study
From: Geographic differences in the distribution of molecular subtypes of breast cancer in Brazil
Antibody to | Clone | Source | Dilution | Epitope retrieval |
|---|---|---|---|---|
Estrogen receptor | Rabbit monoclonal antibody, SP1 | Thermo Scientific | 1:500 | Pressure cooker, 9 min |
Progesterone receptor | Mouse monoclonal antibody, PgR636 | Dako | 1:1000 | Pressure cooker, 9 min |
HER2 | Rabbit monoclonal antibody, SP3 | Thermo Scientific | 1:100 | Microwave oven |
Ki-67 | Mouse monoclonal antibody, MIB1 | Dako | 1:600 | Pressure cooker, 9 min |