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Geographic differences in the distribution of molecular subtypes of breast cancer in Brazil
© Carvalho et al.; licensee BioMed Central Ltd. 2014
Received: 22 June 2014
Accepted: 19 August 2014
Published: 29 August 2014
To compare the distribution of the intrinsic molecular subtypes of breast cancer based on immunohistochemical profile in the five major geographic regions of Brazil, a country of continental dimension, with a wide racial variation of people.
The study was retrospective observational. We classified 5,687 invasive breast cancers by molecular subtype based on immunohistochemical expression of estrogen-receptor (ER), progesterone-receptor (PR), human epidermal growth factor receptor 2 (HER2), and Ki-67 proliferation index. Cases were classified as luminal A (ER and/or PR positive and HER2 negative, Ki-67 < 14%), luminal B (ER and/or PR positive, HER2 negative, and Ki-67 > 14%), triple-positive (ER and/or PR positive and HER2 positive), HER2-enriched (ER and PR negative, and HER2- positive), and triple-negative (TN) (ER negative, PR negative, and HER2- negative). Comparisons of the ages of patients and molecular subtypes between different geographic regions were performed.
South and Southeast regions with a higher percentage of European ancestry and higher socioeconomic status presented with the highest proportion of luminal tumors. The North region presented with more aggressive subtypes (HER2-enriched and triple-negative), while the Central-West region predominated triple-positive carcinomas. The Northeast—a region with a high African influence—presented intermediate frequency of the different molecular subtypes. The differences persisted in subgroups of patients under and over 50 years.
The geographic regions differ according to the distribution of molecular subtypes of breast cancer. However, other differences, beside those related to African ancestry, such as socioeconomic, climatic, nutritional, and geographic, have to be considered to explain our results. The knowledge of the differences in breast cancer characteristics among the geographic regions may help to organize healthcare programs in large countries like Brazil with diverse economic and race composition among different geographic regions.
Breast cancer remains a major health problem responsible for 458,400 deaths worldwide in 2008 . The molecular intrinsic subtypes discovered in 1999 provided additional information on the clinical outcome independent of conventional prognosticators such as tumor size, tumor grade and lymph node status . More importantly, various molecular subtypes respond differently to various chemotherapy treatments, so an accurate subclassification is important for deciding treatment options [3–6].
Racial/ethnic differences have been observed among the different molecular subtypes [7–12] with the most documented being the relatively high incidence of basal type breast cancer (also called “triple negative”) among African-American women compared to Caucasian females [7, 8, 12, 13]. Although non-white women, particularly of African descent, have a lower incidence of breast cancer, this particular race group present with more aggressive tumors and a higher mortality rate [13, 14].
We investigated whether a similar effect of race could be present in Brazil, a country of a continental size with a wide variation in the distribution of people from various racial backgrounds in its five major geographic regions. Brazil is a large country with the fifth largest area and the fifth largest population in the world with a total of more than 190 million inhabitants according to the 2010 official census . It has 26 states divided over five major geographic regions: North, Northeast, Central-West, Southeast and South. The social-economic situation and ethnic backgrounds vary greatly among these regions and include descendants from Amerindians, European immigrants, Asians, and Africans brought to the continent as slaves in the 18th century. Although there is a tendency of one race predominant over another in these regions (for example, Amerindians in the North, blacks in the Northeast and Europeans in the South and Southeast), there are some ethnic variations in the Brazilian population mainly due to a high rate of interracial marriage. Anti-miscegenation and segregation laws have not been part of the Brazilian culture, so Brazil is a home to a population characterized by a color continuum between white and black races. This leads to a well-known difficulty in categorizing races because they lack a precise legal definition .
Previous studies actually demonstrated that in Brazil color, as determined by a physical evaluation, is a poor predictor of genetic ancestry estimated by molecular markers [17, 18]. There remain, however, significant geographical differences in the racial makeup of the population, influenced by diverse geographic and economic characteristics of this country with the size comparable to a continent. The knowledge of possible differences in a large and ethnically complex country such as Brazil clearly would benefit not only the study of breast cancer in this country, but also the comprehension of the mechanisms involved in different molecular subtypes, not to mention the opportunity to develop more efficient strategies of prevention and early detection of breast cancer, particularly among the minorities.
In Brazil, federal law regulates mammographic screening and it has been offered to all women over 50 years of age since 2009. The number of new cases of breast cancer in Brazil estimated in 2014 was 57,120 . Currently, there is no data regarding the distribution of breast cancer molecular subtypes in Brazil or even within individual states of this country. Our aim is to compare the distribution of the intrinsic molecular subtypes by the immunohistochemical surrogates in the five major geographic regions in Brazil.
All patient data were obtained from the files of Consultoria em Patologia, a private surgical pathology laboratory located in the city of Botucatu, State of São Paulo, Brazil. Consultoria em Patologia is a reference laboratory and analyzes over 5,000 breast cancer cases per year. These cases usually are sent by pathologists and oncologists from all five geographic regions of Brazil in order to be evaluated for predictive and prognostic immunohistochemical markers, i.e., estrogen and progesterone receptors (ER and PR), human epidermal growth factor receptor 2 (HER2) and Ki-67 proliferation index. Age at diagnosis and the state of origin of the patients were obtained from the pathological report. Geographic regions of Brazil were classified as North, Northeast, Central-West, South, and Southeast. We selected consecutive cases of invasive breast cancer from July 2009 to March 2011 with assessable immunohistochemical study of ER, PR, HER2 and Ki-67. One of the reasons this period of time was chosen is that all these cases had the immunohistochemistry study performed using exactly the same immunohistochemical protocol. Another reason was that we included only the cases sent for routine prognostic and predictive factors determination. We excluded in situ and microinvasive carcinomas as well the cases, which were sent for second opinion.
Source and dilutions of the antibodies and epitope retrieval methods used in this study
Rabbit monoclonal antibody, SP1
Pressure cooker, 9 min
Mouse monoclonal antibody, PgR636
Pressure cooker, 9 min
Rabbit monoclonal antibody, SP3
Mouse monoclonal antibody, MIB1
Pressure cooker, 9 min
Molecular subtype classification
Immunohistochemical surrogate markers (ER, PR, HER2, Ki-67, EGFR and CK5/6) were used to determine the molecular category of breast cancer as previously described in the following groups: luminal A (ER + and/or PR+, HER2- and Ki-67 < 14%), luminal B (ER + and/or PR+, HER2- and Ki-67 > 14%), triple-positive (TP) (ER + and/or PR+, HER2+), HER2-enriched (ER- and PR- and HER2+), and triple-negative (TN) (ER-, PR- and HER2-) [21, 22]. HER2+ included all the cases with a score of 3+ by immunohistochemistry and cases with score 2+ but showing amplification demonstrated by FISH according to the guidelines of The American Society of Clinical Oncology (ASCO) and the College of American Pathologists (CAP) .
Descriptive statistical analysis was used to characterize the distribution of the patient’s age at diagnosis, hormonal receptor status, HER2 status, and molecular subtypes for the total sample and by the geographic region. Comparisons of the age of patients between different geographic regions, molecular subtypes, ER/PR status, and HER2 status were performed using the Kruskal-Wallis test. Associations between molecular subtypes, hormonal receptor status, HER2 expression, and categories of patient’s age with geographic regions were tested by a chi-square test. Missing values were not included in our statistical analysis and were deleted list-wise. Statistical analyses were performed using MedCalc for Windows (version 188.8.131.52; MedCalc Software, Mariakerke, Belgium), and a p-value less than 0.05 was considered significant.
The study was approved by the Scientific Committee of the Department of Pathology of the Faculdade de Medicina da Universidade de Sao Paulo, and also by the Ethical Committee for Research Projects of the Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo (CAPPesq) (protocol 311/10).
This study has adhered to the STROBE guidelines for observational studies.
Comparison of age of patients at breast cancer diagnosis, molecular subtype, expression of estrogen and progesterone receptor, and HER2 status among the five Brazilian geographic regions
Brazilian geographic regions
(mean ± SD)
56.1 ± 13.5
55.8 ± 13.9
55.3 ± 13.7
54.3 ± 12.8
53.9 ± 13.1
Comparison of age among different geographic regions, molecular subtypes, ER/PR status, and HER2 status
Mean ± SD (years)
Significant difference (<0.05) from other factor(s)a
54.2 ± 12.8
(2) LUM A
58.5 ± 13.0
(3) LUM B
55.7 ± 13.7
53.1 ± 13.4
51.6 ± 13.0
55.6 ± 13.5
53.4 ± 13.4
52.8 ± 12.9
56.1 ± 13.6
Comparison of the molecular subtypes among the five Brazilian geographic regions according to age of patients at diagnosis
Age at diagnosis
94 /810 (11.6%)
238 /669 (35.6%)
90 /262 (34.3%)
Distribution of 5,687 cases compared to overall and female Brazilian population among the five geographic regions, and incidence of breast cancer/100,000 women among females
Total female populationa
Incidence of breast cancer/100,000b
Brazil is a large country with a surface area of 8,511,960 km2 and with a population of 190,755,799. It is the most populous country in Latin America as well as one of the most populous in the world. The female population, according to the official 2010 census, is 97,348,809 . We found significant differences in the proportion of the molecular subtypes of breast cancer between the five Brazilian geographic regions—each one with a distinct racial composition together with differences in climate, nutritional habits, urbanization and socioeconomic conditions.
The Brazilian racial composition is very heterogeneous with most populations descending from Europeans, Africans, Amerindians of various ethnic groups, and Asians. Most importantly, Brazil has experienced a high rate of intermarriage, and consequently, a mixed-race population has been created along five centuries of admixture.
A racial/ethnic influence on the presentation of breast cancer has been suggested in various studies [7, 14, 23–26] although race definition was neither very clear nor uniform in these studies. Most investigated race as a social characteristic without biologic basis of classification. Carey et al. conducted a population-based, case-controlled study with cases from the Carolina Breast Cancer Study and observed a higher prevalence of basal-like among premenopausal African Americans (39%) compared with postmenopausal African Americans (14%) and non-African American patients of any age (16%) . Kurian et al. demonstrated different lifetime risks of the distinct subtypes defined by the ER, PR, and HER2 status among black, Hispanic, Asian, and white women . The authors found a higher lifetime risk of TN tumors in black women and discussed the importance of this data on health policy and resource planning.
The high prevalence of basal-like tumors in premenopausal African Americans is pointed to as an important factor to the poorer prognosis in this group of patients [27, 28], justifying the higher mortality rate despite the lower incidence [13, 14]. Harper et al. documented 31/100,000 annual deaths among African Americans compared with 27/100,000 annual deaths for white women . Delay in diagnosis due to disparities in healthcare access may contribute to higher rates of late-stage presentation and partially explain the increased rate of annual death in African Americans. In this study, we did not evaluate the stage or prognostic or outcome—factors influenced by social-economic status—but only the molecular subtype as a variable in geophysical distribution. In Brazil, besides the complexity of racial composition, the five geographic regions differ from each other regarding climate, urbanization, socioeconomic status, and nutritional habits— conditions that potentially can interfere in carcinogenesis as well as in the diagnosis and outcome of breast cancer.
In our study, Northern Brazil presented the highest proportion of TN carcinomas (20.3%). This region, largely covered by the Amazon rainforest, presents the largest Amerindian influences, both in culture and ethnicity. Indians represent 0.4% of the total Brazilian population , but they make up 1.5% of the Northern region. This region also has the highest African influence (77.8%), compared to 66.8% in Northeast, 65.9% in Central-West, 43.8% in Southeast, and 22.8% in South .
We must consider, however, the difficulty in defining race in Brazil and the need of a genomic classification. Pena et al. estimated the proportion of European, African, and Amerindian ancestry using a panel of 40 validated ancestry-informative insertion-deletion DNA polymorphisms in a population composed by black, white, and brown people from the four most populous Brazilian geographic regions (North, South, Northeast and South) . The results were surprising as the authors were unable to demonstrate statistically significant differences among the four studied regions . The authors found that European ancestry was predominant in all regions, varying from 60.6% in the Northeast to 77.7% in the South. Besides the difficulty to determine race in Brazil, these data show an important difference between the social and genomic determination of the African influence, particularly among the North population. The large area of Brazil has a variety of climate zones: equatorial, tropical, semi-arid, highland tropical and subtropical, each one with different life style, in part determined by the contribution of different ethnical groups, part by the substantial differences in the industrialization degree and social development.
The South region of Brazil houses the largest percentage of European descents, including German, Italian and Polish ancestry. White races comprise 80.6% of the population in this region compared to 24% in the North, 29.5% in the Northeast, 58.5% in the Southeast and 43.5% in the Central-West. According to our results, the South region showed the highest frequency of luminal A carcinomas—the more favorable molecular subtype—as well as a higher frequency of ER/PR-positive tumors, which is consistent with other studies [7, 30, 31]. According to the Brazilian National Institute of Cancer (INCA), Rio Grande do Sul, a state in the Southern region with a great influence of Italians and Germans boasts the highest incidence of breast cancer with 71 cases/100,000 women , which is consistent with other studies . Our results indicated that most of our breast cancers located in this geographic region are a luminal A subtype and simultaneously presented with the lowest rate of HER2, TP, and TN tumors, suggesting an overall better prognosis.
The second region with a high proportion of luminal A and ER/PR-positive carcinomas was the Southeast, which was also the region with the second largest white population. The lowest incidence of luminal A was documented in the North, which presented a rate of breast cancer varying from 10 cases/100,000 women in the state of Acre to 26 cases/100,000 women in the state of Rondonia . According to INCA, the only state of the North with a discrepant higher rate of breast cancer is Tocantins, which is located near the Central-West region, with 27 cases/100,000 women .
In addition to luminal A carcinomas, the luminal B category also was more prevalent in the South and Southeast and showed a lower frequency in the North region. It is important to stress that our luminal B did not include HER2-coexpression, like some authors have classified [7, 11]. We analyzed the TP phenotype separately using classification criteria suggested by the previous St. Gallen consensus . The TP phenotype was more frequent in the Central-West region, while HER2-enriched carcinomas were more prevalent in the North. In fact, our results indicated that more aggressive molecular subtypes are more prevalent in the North. This information must be considered in the planning of breast cancer prevention programs.
The distribution of HER2 carcinomas among different races/ethnic groups is more difficult to analyze because some authors included TP within the HER2 group , and others, within luminal B [7, 11, 13, 30]. Fewer studies analyzed the group separately as we did. Kurian et al. did not find differences in the distribution of HER2- carcinomas, but they included TP among their HER2 tumors .
The differences in molecular subtypes that we observed among the five geographic regions persisted in the subgroups of patients under and over 50 years, suggesting the existence of a geographic phenomenon more than effect of age itself. Similar results have been reported by others . Clarke et al. analyzed the distribution of the breast cancer subtypes among 91,908 patients from California and observed that black-women had higher rates of triple-negative carcinomas at all ages. The North region presented the lowest mean of age, and it was statistically different when compared to the Northeast, South, and Southeast.
Although we found significant differences in the distribution of molecular subtypes between the five geographic regions, we could not associate them to African ancestry, as other studies have been able to do in primarily the USA, despite the important African influence in the Brazilian racial composition. The reasons for this finding remain to be explored. One possible explanation is that the influence of African ancestry in Brazil may be different from that seen in the USA and Europe due to high rate of interracial marriage seen in Brazil. However, as we discussed above, there are other important factor to be considered,
There are studies that explored some genetic conditions implicated with racial/ethnic differences in breast cancer. Wang et al. observed a significantly higher methylation of the suppressor gene CDH13 in breast tumor samples from African-American women when compared with European-American patients. The hypermethylation of the CDH13 gene probably is related to early onset ER-negative breast cancer . Several single-nucleotide polymorphisms (SNPs) have been associated to age and race of breast cancer patients . Associations were observed for SNPs in FGFR2, LSP1, H19, TLR1/TLR6 and RELN for African-Americans and in FGFR2, TNRC9, H19 and MAP3K1 for whites . It is highly probable that the racial admixture can be responsible for some genomic differences, which are not necessarily similar to the ancestry.
Health disparities, either by racial/ethnic, socioeconomic, cultural, climatic, nutritional, or geographic are very complex to decode since there is a significant overlap between these factors . Understanding the differences in breast cancer characteristics between geographic regions is the first step to organize healthcare programs. Moreover, this knowledge has important impact for the design and interpretation of clinical trials. As such, we believe that our study is relevant to determining the best strategy of health care and to better understand the tumor biology of breast cancer in large countries like Brazil with diverse economic and race composition among different geographic regions.
The distribution of molecular subtypes of breast cancer differs by geographic region in Brazil, a country of continental dimension and a wide racial variation of people. These differences are multifactorial and must be taken into account for public health policy.
We thank Dr. Robert West who revised the manuscript.
There was no external scientific (medical) writer involved in the preparation and editing of this manuscript.
None of the authors received any funding related the collection, analysis, and interpretation of data; in the writing of the manuscript; or in the decision to submit the manuscript for publication.
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